Anti-Citrate synthetase antibody [2H8BB6] (ab128564)
Key features and details
- Mouse monoclonal [2H8BB6] to Citrate synthetase
- Suitable for: IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Citrate synthetase antibody [2H8BB6]
See all Citrate synthetase primary antibodies -
Description
Mouse monoclonal [2H8BB6] to Citrate synthetase -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human Liver Mitochondria;
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
2H8BB6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunoprecipitation using ab128564
Lane 1: Ladder
Lane 2: Human Liver Mitochondria -
Immunofluorescent staining of Citrate synthetase in Hela cells (human) using ab128564.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128564, 5µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.