Hexokinase Activity Assay Kit (Fluorometric) (ab211103)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 2 µU
Overview
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Product name
Hexokinase Activity Assay Kit (Fluorometric)
See all Hexokinase kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
2 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
The Hexokinase Activity Assay Kit (Fluorometric) (ab211103) provides a simple, sensitive and quick method for monitoring hexokinase (HK) activity in cells, serum, and animal or plant tissues. In this assay, HK converts glucose into glucose-6-phosphate, which in turn undergoes a series of reactions and reduces the sensitive probe to generate an intense fluorescent product that can be easily detected at Ex/Em = 535/587 nm.
This assay can detect as low as 2 µU of HK activity.
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Notes
Hexokinase (HK, 6-Phosphate glucose kinase, ATP:D-Hexose 6- Phosphotransferase, ATP-dependent hexokinase, EC 1.1.1.49) is responsible for phosphorylating hexoses (six-carbon sugars) to form hexose phosphate. Hexokinases play an important role in glucose metabolism, as glucose is the most important substrate of hexokinases. Hexokinases are found in many organisms including bacteria, plants and mammals. In mammals, there are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III are referred as “low Km” because of their high affinity for glucose (Km
Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ATP (50 µmol) 1 vial HK Assay Buffer 1 x 25ml HK Developer (50 U) 1 vial HK Enzyme (100 U) 1 vial HK Positive Control (500 mU) 1 vial HK Substrate 1 x 1ml NADPH Standard (200 nmol) 1 vial PicoProbe (in DMSO) 1 x 400µl -
Research areas
Images
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Hexokinase Activity Assay Kit (Fluorometric) (ab211103)
Typical NADPH standard calibration curve.
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Hexokinase Activity Assay Kit (Fluorometric) (ab211103)Kinetic curves showing Hexokinase activity in positive control (included in kit), lysates from HeLa (0.62 µg), Jurkat (0.65 µg) and MCF-7 cells (0.56 µg), and lysates from orchid leaf (4 µg).
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Hexokinase Activity Assay Kit (Fluorometric) (ab211103)Hexokinase specific activity in lysates from orchid leaf (4 µg), MCF-7 (0.56 µg), Jurkat (0.65 µg) and HeLa cells (0.62 µg).
