Anti-MCM3 antibody (ab272877)
Key features and details
- Rabbit polyclonal to MCM3
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM3 antibody
See all MCM3 primary antibodies -
Description
Rabbit polyclonal to MCM3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human MCM3. Center region.
Database link: P25205 -
Positive control
- WB: HepG2 whole cell lysate. IHC-P: Mouse colon tissue. ICC/IF: HeLa cells. IP: HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM3 antibody (ab272877)Anti-MCM3 antibody (ab272877) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
7.5% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (ab272877)Paraffin-embedded mouse colon tissue stained for MCM3 using ab272877 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody (ab272877)4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCM3 using ab272877 at 1/500 dilution in ICC/IF.
Red: alpha Tubulin, a cytoskeleton marker.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-MCM3 antibody (ab272877)Samples: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
A. 40 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM3 protein by 4 μg of ab2728777.5 % SDS-PAGE
The immunoprecipitated MCM3 protein was detected by ab272877 diluted at 1:1000, followed by anti-rabbit IgG.