Anti-Hexokinase II antibody (ab227198)
Key features and details
- Rabbit polyclonal to Hexokinase II
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hexokinase II antibody
See all Hexokinase II primary antibodies -
Description
Rabbit polyclonal to Hexokinase II -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment within Human Hexokinase II (internal sequence). The exact sequence is proprietary.
Database link: P52789 -
Positive control
- WB: Mouse brain tissue lysate; U087 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extract. IHC-P: U-87 xenograft tissue; rat kidney, stomach and heart tissue; mouse muscle tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution (HRP-conjugated anti-rabbit IgG antibody) + Mouse brain tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 102 kDa7.5% SDS PAGE
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Paraffin-embedded rat kidney tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
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Ice cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hexokinase II (green) using ab227198 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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All lanes : Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 102 kDa7.5% SDS-PAGE
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Paraffin-embedded U87 (human) xenograft tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse muscle tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat heart tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat stomach tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.