Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free
    See all MGEA5/OGA primary antibodies

  • Description

    Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab155521)

  • Positive control

    • IHC-P: Human kidney and colon tissues. IP: HeLa cells. WB: HeLa, JAR, and HEK-293T cell lysates.

  • General notes

    ab227989 is the carrier-free version of ab124807 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab227989 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Previously labelled as MGEA5

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    All lanes : Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MGEA5 knockout HeLa cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab124807).

    Lanes 1-4: Merged signal (red and green). Green - ab124807 observed at 130 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab124807 Anti-MGEA5/OGA antibody [EPR7154(B)] was shown to specifically react with MGEA5/OGA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265821 (knockout cell lysate ab257263) was used. Wild-type and MGEA5/OGA knockout samples were subjected to SDS-PAGE. ab124807 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MGEA5/OGA with purified ab124807 at 1:50 dilution (12.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)

    ab124807 (purified) at 1:30 dilution (2µg) immunoprecipitating MGEA5/OGA in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab124807 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124807 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124807).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)

    This IHC data was generated using the same anti-MGEA5/OGA antibody clone, EPR7154(B), in a different buffer formulation (cat# ab124807).

    ab124807, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)
    Anti-MGEA5/OGA antibody [EPR7154(B)] - BSA and Azide free (ab227989)

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