Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGEA5/OGA antibody [EPR7154(B)]
    See all MGEA5/OGA primary antibodies

  • Description

    Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MGEA5/OGA. The exact sequence is proprietary.
    (Peptide available as ab155521)

  • Positive control

    • WB: HEK-293T, HeLa, BxPC-3, JAR and fetal brain lysates. IHC-P: Human kidney and colon tissue. IP: HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Previously labelled as MGEA5.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    All lanes : Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MGEA5 knockout HeLa cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab124807 observed at 130 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab124807 Anti-MGEA5/OGA antibody [EPR7154(B)] was shown to specifically react with MGEA5/OGA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265821 (knockout cell lysate ab257263) was used. Wild-type and MGEA5/OGA knockout samples were subjected to SDS-PAGE. ab124807 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MGEA5/OGA with purified ab124807 at 1:50 dilution (12.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 20ug at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)

    ab124807 (purified) at 1:30 dilution (2µg) immunoprecipitating MGEA5/OGA in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab124807 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124807 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    All lanes : Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : BxPC-3 lysate
    Lane 3 : JAR lysate
    Lane 4 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)

    ab124807, at 1/100 dilution, staining MGEA5/OGA in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
    Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)

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