Anti-PFKFB4 antibody (ab137785)
Key features and details
- Rabbit polyclonal to PFKFB4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PFKFB4 antibody
See all PFKFB4 primary antibodies -
Description
Rabbit polyclonal to PFKFB4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human PFKFB4 aa 200-469 (internal sequence). The exact sequence is proprietary.
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Positive control
- WB: HepG2 and Raji cell lysates; IHC-P: NCI-N87 xenograft, mouse spleen and testis tissue; ICC/IF: NT2D1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with a citrate buffer, pH 6.0, 15 min
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Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (ab137785)Immunocytochemistry/ Immunofluorescence of NT2D1 staining PFKFB4 using ab137785 at a dilution of 1/500. Nuclear staining using Hoechst 33342.
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Western blot - Anti-PFKFB4 antibody (ab137785)Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution + Rat tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 54 kDa
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Western blot - Anti-PFKFB4 antibody (ab137785)Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min
