Anti-ANKRD23 antibody (ab122320)
Key features and details
- Rabbit polyclonal to ANKRD23
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKRD23 antibody -
Description
Rabbit polyclonal to ANKRD23 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: KRLRHRVPPR KPEPLVKPQS QAQVEPVGLE MFLKAAAENQ EYLIDKYLTD GGDPNAHDKL HRTALHWAC, corresponding to amino acids 84-152 of Human ANKRD23 (Q86SG2).
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Positive control
- ICC: A-431 cells. IHC: human smooth muscle and prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (ab122320)
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human prostate with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (ab122320) -
Western blot - Anti-ANKRD23 antibody (ab122320)All lanes : Anti-ANKRD23 antibody (ab122320) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
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Immunocytochemistry/ Immunofluorescence - Anti-ANKRD23 antibody (ab122320)Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization: PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.
