Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 03, 2021

Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18289] to PGC1 alpha + beta
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-PGC1 alpha + beta antibody [EPR18289]
See all PGC1 alpha + beta primary antibodies
Description

Rabbit monoclonal [EPR18289] to PGC1 alpha + beta
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

Recombinant fragment

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant fragment of Human PGC1 alpha protein; Recombinant fragment of Human PGC1 beta protein; Human skeletal muscle lysate, HeLa cell lysate and mouse colon lysate; Human fetal heart lysate, Human fetal kidney lysate, Mouse thymus lysate and Rat thymus lysate; Mouse brain and heart lysates; Rat brain and heart lysates; C6 and NIH/3T3 cell lysates; Mouse kidney lysate and rat kidney lysate; Human skeletal muscle lysate.
General notes
This product may be covered by one or more of the following U.S. Patents.

6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Mouse Rat Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18289
Isotype

IgG
Research areas

Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag®.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag®.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse colon lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 90, 113 kDa why is the actual band size different from the predicted?

5% NFDM/TBST: Blocking and diluting buffer.
The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925).
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Mouse thymus lysate
Lane 4 : Rat thymus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

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