Anti-Alpha-synuclein (phospho Y125) antibody (ab131466)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y125)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Alpha-synuclein (phospho Y125) antibody
See all Alpha-synuclein primary antibodies -
Description
Rabbit polyclonal to Alpha-synuclein (phospho Y125) -
Host species
Rabbit -
Specificity
ab131466 detects alpha Synuclein only when phosphorylated at tyrosine 125. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Alpha-synuclein conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH, surrounding phosphorylation site of Tyrosine 125 (E-A-Yp-E-M) derived from Human alpha Synuclein (NP_000336.1).
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Positive control
- HeLa cells; extracts of C2C12 cells treated with anisomycin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131466 was purified by affinity- chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Alpha-synuclein (phospho Y125) antibody (ab131466)All lanes : Anti-Alpha-synuclein (phospho Y125) antibody (ab131466) at 1/500 dilution
Lane 1 : Extract from C2C12 cells, untreated
Lane 2 : Extract from C2C12 cells treated with Anisomycin
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y125) antibody (ab131466)Immunoflourescent analysis of alpha Synuclein (phospho Y125)in methanol fixed HeLa cells stained with ab131466 at a 1/100 dilution.
