Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

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Anti-VEGFD antibody [EPR8457] (ab155288)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8457] to VEGFD
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-VEGFD antibody [EPR8457]
See all VEGFD primary antibodies
Description

Rabbit monoclonal [EPR8457] to VEGFD
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human VEGFD. The exact sequence is proprietary.
Database link: O43915
(Peptide available as ab189506)
Positive control
- Raji, HT-1080, MCF-7, and Caco-2 cell lysates. Human heart and lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8457
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control: PBS in place of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab155288 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution

Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
Lane 2 : HT1080 (human fibrosarcoma) whole cell lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

Diluting and blocking buffer: 5% NFDM/TBST
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution (Unpurified) + GST-Tagged human VEGFD (aa 1 to 354) recombinant protein

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 40 kDa
Observed band size: 65 kDa
why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
Flow Cytometry - Anti-VEGFD antibody [EPR8457] (ab155288)

ab155288 staining VEGFD in the mouse cell line NIH/3T3 (mouse embryo) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/2000 dilution

Lane 1 : Rat lung tissue lysate
Lane 2 : NIH/3T3 (mouse embryo) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

Diluting and blocking buffer: 5% NFDM/TBST
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)

All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/1000 dilution

Lane 1 : Raji lysate
Lane 2 : HT-1080 lysate
Lane 3 : MCF-7 lysate
Lane 4 : Caco-2 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155288 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab155288showing +ve staining.
Anti-VEGFD antibody [EPR8457] (ab155288)

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