Anti-VEGFD antibody (ab103685)
Key features and details
- Rabbit polyclonal to VEGFD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VEGFD antibody
See all VEGFD primary antibodies -
Description
Rabbit polyclonal to VEGFD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human VEGFD aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab119174) -
Positive control
- This antibody gave a positive signal in HT1080 whole cell lysate as well as the following tissue lysates: Human Heart; Human Fetal Lung; Human Colon.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications Macaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).IHC-P Use a concentration of 10 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).IHC-P
Use a concentration of 10 µg/ml.Target
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Function
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
Tissue specificity
Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Post-translational
modificationsUndergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2277 Human
- Omim: 300091 Human
- SwissProt: O43915 Human
- Unigene: 11392 Human
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Alternative names
- c-fos induced growth factor antibody
- c-fos induced growth factor (vascular endothelial growth factor D) antibody
- c-fos induced growth factor antibody
see all
Images
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Western blot - Anti-VEGFD antibody (ab103685)All lanes : Anti-VEGFD antibody (ab103685) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human Fetal Heart - Total Protein Tissue Lysate
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 140 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody (ab103685)IHC image of VEGFD staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103685, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (1)
ab103685 has been referenced in 1 publication.
- Kim HJ et al. Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer. Ann Coloproctol 34:88-93 (2018). PubMed: 29742856
Images
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Western blot - Anti-VEGFD antibody (ab103685)All lanes : Anti-VEGFD antibody (ab103685) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human Fetal Heart - Total Protein Tissue Lysate
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 140 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody (ab103685)
IHC image of VEGFD staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103685, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
