Recombinant rat VEGFA protein (ab92846)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant rat VEGFA protein
See all VEGFA proteins and peptides -
Biological activity
Biological Activity: As determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) when compared to a standard. The expected ED50 is 4.0-8.0 ng/ml. -
Purity
> 95 % SDS-PAGE.
Purity is approximately 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.165% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Images
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Functional Studies - Recombinant rat VEGFA protein (ab92846)Functional analysis of ab92846
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SDS-PAGE - Recombinant rat VEGFA protein (ab92846)SDS PAGE analysis of ab92846 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant rat VEGFA protein (ab92846)ab92846 used in Functional Studies.
