Recombinant human VEGFA protein (Active) (ab155740)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
Normally Mannitol or Trehalose are added as protectants before lyophilisation.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human VEGFA protein (ab155740)
Avastin (Bevacizumab) captured on CM5 chip via ant-human IgG Fc antibodies surface, can bind ab155740 with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200).
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SDS-PAGE - Recombinant human VEGFA protein (ab155740)ab155740 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
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Functional Studies - Recombinant human VEGFA protein (ab155740)A SEC-HPLC analysis showing 95% of ab155740 present as active homodimers.
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Functional Studies - Recombinant human VEGFA protein (Active) (ab155740)Immobilized ab155740 at 2 µg/mL (100 µL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 µg/mL (100 µL/well) Biotinylated Human VEGF R2 protein with a half maximal inhibitory concentration (IC50) of 0.70 µg/mL.
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Functional Studies - Recombinant human VEGFA protein (Active) (ab155740)Immobilized ab155740 at 2 µg/mL (100 µL/well) can bind Biotinylated Human VEGF R2 protein with a linear range of 10-156 ng/mL.
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Functional Studies - Recombinant human VEGFA protein (Active) (ab155740)Immobilized ab155740 at 0.01 µg/mL (100 µL/well) can bind Human VEGF R1 protein with a linear range of 4-31 ng/mL.
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Functional Studies - Recombinant human VEGFA protein (ab155740)Inhibition assay shows that the proliferation effect of ab155740 is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/ml. The ED50 is 0.065-0.229 µg/mL.
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Functional Studies - Recombinant human VEGFA protein (ab155740)ab155740 stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/ml.
