Recombinant human VEGFA protein (ab53824)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
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Product name
Recombinant human VEGFA protein
See all VEGFA proteins and peptides -
Biological activity
The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF has been determined to be in the range of 10 ng/ml.
Specific activity: 2.5 x 105 units/mg.
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Purity
> 95 % SDS-PAGE.
Purity: 95%, by SDS-PAGE and visualised by silver stain Endotoxin level: -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
None
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThe lyophilised VEGF is soluble in water and most aqueous buffers. It should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
Images
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Functional Studies - Recombinant human VEGFA protein (ab53824)VEGF145-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC). HDLECs were stimulated with increasing amounts of human VEGF145.
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SDS-PAGE - Recombinant human VEGFA protein (ab53824)SDS-PAGE analysis of recombinant human VEGF-A isoforms produced in E. coli. Samples were loaded under non-reducing conditions in 15% SDS-polyacrylamide gel and stained with Silver stain. -
SDS-PAGE - Recombinant human VEGFA protein (ab53824)SDS-PAGE page analysis of ab53824
