Recombinant human VEGF189 protein (ab106307)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituent: 0.3% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionShould be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
Images
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Functional Studies - Recombinant human VEGF189 protein (ab106307)
VEGF-A Sandwich-ELISA using VEGF189 as standard. Mouse anti-human VEGF-A was used as capture antibody, Biotinylated mouse anti-human VEGF-A was used for detection.
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SDS-PAGE - Recombinant human VEGF189 protein (ab106307)SDS-PAGE analysis of recombinant human VEGF-A isoforms produced in E. coli. Samples were loaded under non-reducing conditions in 15% SDS-polyacrylamide gel and stained with Silver stain. -
Functional Studies - Recombinant human VEGF189 protein (ab106307)VEGF189-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC). HDLECs were stimulated with increasing amounts of recombinant human VEGF189.