Human VEGF-165 ELISA Kit (ab273164)
Key features and details
- Sensitivity: 15.9 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGF-165 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Various 3.8% Inter-assay Sample n Mean SD CV% Various 7.6% -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.9 pg/ml -
Range
31.25 pg/ml - 1000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human VEGF165 ELISA kit (ab273164) is for the in-vitro qualitative and quantitative determination of VEGF165 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human VEGF165.
A capture antibody highly specific for VEGF-A has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of VEGF165 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-VEGF165 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of VEGF165 present in the samples and standards.
The absorbance of the colour complex is measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of VEGF165 in any sample tested.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody VEGF-165 1 x 400µl HRP Diluent 1 x 23ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 25ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml VEGF-165 Coated Microwell strips 1 unit VEGF-165 Standard 2 vials Wash Buffer 1 x 10ml -
Research areas
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Cellular localization
VEGF 165A: Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. -
Database links
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: P15692-8 Human
- Unigene: 73793 Human
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of HumanVEGF165 ELISA kit standard curve.