Human VEGFB ELISA Kit (ab277458)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGFB ELISA Kit
See all VEGFB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 90.69 70% - 118% Plasma 104.8 69% - 149% Cell culture media 103.6 71% - 137% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human VEGFB ELISA Kit (ab277458) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGFB in serum (human VEGFB concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human VEGFB coated on a 96-well plate. Standards and samples are pipetted into the wells and Human VEGFB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human VEGFB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human VEGFB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human VEGFB Antibody 2 vials Anti-Human VEGFB coated Microplate 1 unit Human VEGFB standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. -
Tissue specificity
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Post-translational
modificationsVEGF-B186 is O-glycosylated. -
Cellular localization
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. - Information by UniProt
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Alternative names
- Vascular endothelial growth factor B
- Vascular endothelial growth factor-related factor
- VEGF B
see all -
Database links
- Entrez Gene: 7423 Human
- Omim: 601398 Human
- SwissProt: P49765 Human
- Unigene: 78781 Human
- Unigene: 732095 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.