Recombinant human NEK7 protein (ab196067)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 72% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human NEK7 protein
See all NEK7 proteins and peptides -
Biological activity
Specific Activity: 47 pmol/min/µg
Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and ab196067 for 50 min at 30°C.
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Purity
>= 72 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYR AACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYAS FIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALE HMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTP YYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKI EQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTA SS -
Predicted molecular weight
61 kDa including tags -
Amino acids
1 to 302 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_133494.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human NEK7 protein (ab196067)
4-20% SDS-PAGE analysis of 2.98 μg ab196067 followed by Coomassie staining.
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Functional Studies - Recombinant human NEK7 protein (ab196067)Kinase assay using ab196067 showing specific activity as 47 pmol/min/µg.
