Rabbit specific HRP/AEC (ABC) Detection IHC Kit (ab64260)
Overview
-
Product name
Rabbit specific HRP/AEC (ABC) Detection IHC Kit
See all HRP/AEC Detection Kit kits -
Product overview
Rabbit specific HRP/AEC (ABC) IHC Detection Kit ab64260 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC. -
Notes
For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Mouse / anti-Rabbit HRP-AEC IHC kit ab93705
- Anti-Mouse HRP-AEC IHC Kit ab64258For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468
Alternatively, see HRP-DAB IHC detection kits.Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
-
Tested applications
Suitable for: IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml AEC Single Solution 1 x 15ml Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
-
Relevance
An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate. -
Alternative names
- carbazole
- Horseradish Peroxidase
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Rabbit HRP/AEC Detection Kit (ab64260)Human tonsil stained with anti-CD3 antibody using Anti Rabbit HRP/AEC Detection Kit (ab64260)
