Anti-LKB1 (phospho S428) antibody (ab63473)
Key features and details
- Rabbit polyclonal to LKB1 (phospho S428)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-LKB1 (phospho S428) antibody
See all LKB1 primary antibodies -
Description
Rabbit polyclonal to LKB1 (phospho S428) -
Host species
Rabbit -
Specificity
ab63473 detects endogenous levels of LKB1 only when phosphorylated at serine 428. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LKB1 (phospho S428) antibody (ab63473)All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution
Lane 1 : mouse brain extracts
Lane 2 : mouse brain extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (ab63473)ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
