Mouse MBL ELISA Kit (ab208343)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.5 pg/ml
- Range: 39.063 pg/ml - 2500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MBL ELISA Kit
See all Mannan Binding Lectin/MBL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 5 7.3% Inter-assay Sample n Mean SD CV% Mouse serum 4 14% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
8.5 pg/ml -
Range
39.063 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 79.5 75% - 86.8% Cell culture media 99.7 95.4% - 103.3% Hep Plasma 96.6 88.8% - 101.2% EDTA Plasma 94.9 74.1% - 102.3% Cit plasma 102.7 90.8% - 117.3% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse MBL ELISA Kit (ab208343) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MBL protein in cell culture supernatant, serum, plasma, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MBL with 8.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MBL is a secreted calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MBL Capture Antibody 1 x 600µl 10X Mouse MBL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse MBL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- COLEC 1
- COLEC1
- Collectin-1
see all -
Database links
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
Images
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Other - Mouse MBL ELISA Kit (ab208343)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MBL standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse MBL standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MBL in mouse serum and plasma samplesThe concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: serum 1:83, plasma (citrate) 1:83, plasma (EDTA) 1:167, plasma (heparin) 1:167. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 169.0 ng/mL in serum, 116.5 ng/mL in plasma (citrate), 179.8 ng/mL in plasma (EDTA) and 148.4 ng/mL in plasma (heparin).
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Interpolated concentrations of native MBL in mouse liver supernatant samplesMouse liver cells were cultured for 5 days in a medium and supernatant sample was prepared. The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 4.3 ng/mL in liver supernatant.
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Interpolated concentrations of native MBL in mouse liver extract samples based on a 500 µg/mL extract loadThe concentrations of MBL were measured in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 1.56 ng/mL in liver extract sample.
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Interpolated concentrations of native MBL in mouse liver and testis extract samplesThe concentrations of MBL were measured in three different dilutions in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of MBL per mg of extract (mean +/- SD, n=3).
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Sandwich ELISA - Mouse MBL ELISA Kit (ab208343)To learn more about the advantages of recombinant antibodies see here.
