Human Complement C8 Gamma ELISA Kit (ab222871)
Key features and details
- Sensitivity: 0.6 ng/ml
- Range: 0.875 ng/ml - 56 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C8 Gamma ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 1 20 4.1% Plasma 2 20 3.8% Plasma 3 20 3.9% Inter-assay Sample n Mean SD CV% Plasma 1 20 8% Plasma 2 20 8.2% Plasma 3 20 7.5% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.6 ng/ml -
Range
0.875 ng/ml - 56 ng/ml -
Recovery
99 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Cow, Dog, Pig -
Product overview
Human Complement C8 Gamma ELISA Kit (ab222871 is designed for the quantitative measurement of Complement C8 Gamma (C8G) in human plasma, serum, milk, saliva, cerebrospinal fluid and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures C8G in 4 hours. A polyclonal antibody specific for C8G has been pre-coated onto a 96-well microplate with removable strips. C8G in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C8G, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Complement component 8 (C8) is a 150-kDa complex composed of three genetically distinct subunits: C8 alpha (64 kDa), C8 beta (64 kDa), and C8 gamma (22 kDa). C8 alpha and C8 beta are highly homologous to each other and to C6, C7, and C9. It also contains a common membrane attack complex/perforin (MACPF) domain. C8 gamma has a lipocalin fold and shares no homology with any other complement protein. C8 plays a central role in membrane attack complex (MAC) assembly by coordinating the interaction with complement proteins C5b-7 and the pore-forming protein C9 on pathogen membranes. It is also the first component to penetrate the lipid bilayer.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C8G Antibody 1 x 120µl Anti- Human Complement C8G coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human Complement C8G Standard 2 vials Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C8C
- C8G
- CO8G_HUMAN
see all -
Database links
- Entrez Gene: 733 Human
- Omim: 120930 Human
- SwissProt: P07360 Human
- Unigene: 1285 Human
Images
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Human Complement C8 Gamma (ab222871)
Example of human Complement C8 gamma standard curve.