Mouse MBL Matched Antibody Pair Kit (ab219525)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Mouse MBL Matched Antibody Pair Kit
See all Mannan Binding Lectin/MBL kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.73 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MBL Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MBL.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208343. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MBL Capture Antibody 2 x 50µg 1 x 50µg Mouse MBL Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse MBL Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- COLEC 1
- COLEC1
- Collectin-1
see all -
Database links
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
Images
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Mouse MBL standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse MBL Matched Antibody Pair Kit (ab219525)To learn more about the advantages of recombinant antibodies see here.
