Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
![Anti-LKB1 antibody [Ley 37D/G6] (ab15095)](https://www.abcam.com/ps/products/15/ab15095/Images/ab15095-254244-anti-lkb1-antibody-ley-37d-g6-western-blot.jpg "Anti-LKB1 antibody [Ley 37D/G6] (ab15095)")
Key features and details
- Mouse monoclonal [Ley 37D/G6] to LKB1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-LKB1 antibody [Ley 37D/G6]
See all LKB1 primary antibodies -
Description
Mouse monoclonal [Ley 37D/G6] to LKB1 -
Host species
Mouse -
Specificity
This antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to LKB1.
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Positive control
- HAP1 and MEF cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Ley 37D/G6 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)](https://www.abcam.com/ps/products/15/ab15095/Images/ab15095-264645-anti-lkb1-antibody-ley-37d-g6-western-blot.png)
Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095) Image courtesy of anonymous abreviewerAll lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution
All lanes :
Secondary
All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.
Predicted band size: 48.6 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Negative control (Lane 1): LKB1 knockout MEF cells.
Positive control (Lane 2): wildtype MEF cells.
Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.
Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.
