Human CD48 ELISA kit (ab215419)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human CD48 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1380pg/ml 2.4% 2 16 420pg/ml 3.2% 3 16 170pg/ml 2.9% 4 16 100pg/ml 4.2% Inter-assay Sample n Mean SD CV% 1 3 1293pg/ml 6.97% 2 3 410pg/ml 2.44% 3 3 173pg/ml 3.33% 4 3 100pg/ml 0% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 107.33 25pg/ml - 1600pg/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Product overview
The human CD48 ELISA kit (ab215419) is deigned for the quantitative determination of human CD48 in cell culture supernatant, serum and plasma.
-
Notes
The human CD48 test is based on the quantitative sandwich enzyme immunoassay technique. Microplate wells are pre-coated with anti-human CD48-specific polyclonal capture antibodies. Samples and standards are pipetted into microwells and human CD48 molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human CD48-specific polyclonal detection antibodies bind to different epitopes of human CD48 molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the color produced is directly proportional to the amount of human CD48 in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Anti-Human CD48 coated microplate (12 x 8 wells) 1 unit Human CD48 sample diluent 1 x 25ml Human CD48 standard A (0 pg/mL) 1 x 1ml Human CD48 standard B (25 pg/mL) 1 x 1ml Human CD48 standard C (50 pg/mL) 1 x 1ml Human CD48 standard D (100 pg/mL) 1 x 1ml Human CD48 standard E (200 pg/mL) 1 x 1ml Human CD48 standard F (400 pg/mL) 1 x 1ml Human CD48 standard G (800 pg/mL) 1 x 1ml Human CD48 standard H (1600 pg/mL) 1 x 1ml Human CD48 enzyme conjugate stock 1 x 30µl Human CD48 enzyme conjugate stock diluent 1 x 12ml 10X Wash concentrate 1 x 50ml TMB Substrate solution 1 x 12ml Stop solution 1 x 12ml -
Research areas
-
Function
Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Alternative names
- Antigen CD48
- B cell membrane protein
- B lymphocyte activation marker BLAST 1
see all -
Database links
- Entrez Gene: 962 Human
- Omim: 109530 Human
- SwissProt: P09326 Human
- Unigene: 243564 Human
Images
-
Human CD48 ELISA kit ab215419 Standard Curve.
Cubic spline fit curve on a log-log scale.
-
Human CD48 ELISA kit ab215419 Standard Curve.Linear regression (LLS) fit curve on a lin-lin scale.