Glycerol-3-Phosphate Assay Kit (Fluorometric) (ab252891)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Homogenate
Overview
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Product name
Glycerol-3-Phosphate Assay Kit (Fluorometric)
See all glycerol-3-phosphate kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Homogenate -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
Glycerol-3-Phosphate Assay Kit (Fluorometric) ab252891 is a sensitive, fast and easy-to-use method of measuring Glycerol-3-Phosphate (G3P) concentration in various biological samples. In this assay, G3P is converted into an intermediate by the G3P Enzyme Mix. The G3P developer then utilizes the intermediate to generate a fluorometric signal with a Probe, which can be used to calculate the concentration of G3P in the sample. This assay kit can detect G3P as little as 20 pmol/well and can be used for a variety of sample types.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests G3P Assay Buffer 1 x 25ml G3P Developer 1 vial G3P Enzyme Mix 1 vial G3P Standard 1 vial Probe (in DMSO) 1 x 400µl -
Relevance
Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol- 3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis. -
Alternative names
- G3P
- glycerol 3 phosphate
- glycerol-3-phosphate
Images
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Example Data
Glycerol-3-Phosphate standard curve.
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Example dataGlycerol-3-Phosphate content in pooled human serum. Serum was deproteinized by filtration through a 10 K MWCO spin column and 20 µL of the filtrate was assayed in triplicate according to the kit protocol.
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Example DataGlycerol-3-Phosphate determination in rat liver lysate (10 µg added per well). 100 mg of wet tissue was homogenized in 1 mL assay buffer using a dounce tissue homogenizer and deproteinized by filtration through a 10 kDa MWCO spin column.