TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP) (ab183288)
Overview
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Product name
TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP) -
Assay type
Polymer Multiplex -
Product overview
Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 24 ml 72 ml 240 ml — Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml Blocker A 1 x 12ml 2 x 18ml 1 x 120ml Blocker B 1 x 12ml 2 x 18ml 1 x 120ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Plastic pipette 1 unit 1 unit 1 unit Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rabbit HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml -
Research areas
