Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies, WB
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Product name
Recombinant HIV1 Reverse Transcriptase protein (Active) -
Biological activity
5,000 units/ml.
Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
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Purity
> 90 % SDS-PAGE.
(CBB staining) -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Additional sequence information
Composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles. (GenBank AAA44988.1)
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 6
Constituents: 0.1% Triton-X-100, 0.05% Magnesium chloride, 0.63% Tris HCl, 50% Glycerol, 0.29% Sodium chloride, 0.0154% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant HIV1 Reverse Transcriptase protein (ab63979)
Polyacrylamide gel electrophoresis of ab63979.
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Western blot - Recombinant HIV1 Reverse Transcriptase protein (ab63979)All lanes : Anti-HIV1 Reverse Transcriptase antibody [39/4.12.2] (ab9066) at 1/2000 dilution
Lane 1 :Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.04 µg
Lane 2 :Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.02 µg
Lane 3 :Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.004 µg
Lane 4 :Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.002 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.