Recombinant fdhA protein (ab198463)
Key features and details
- Expression system: Escherichia coli
- Purity: > 74% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant fdhA protein -
Biological activity
Specific Activity: ≥35 µmol/min/µg
Assay Conditions: The reaction was monitored by measuring NADH production by following absorbance at 340nm at room temperature for 5min. Buffer: pH 7.3, 20 mM Tris, 50 mM NaCl, 20 µM Fe2+, 500 mM a-ketoglutarate, 2 mM ascorbic acid, 0.01% Tween-20, and 1 mM NAD+. -
Purity
> 74 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Bacteria -
Sequence
MHHHHHHSGNRGVVYLGSGKVEVQKIDYPKMQDPRGKKIEHGVILKVVST NICGSDQHMVRGRTTAQVGLVLGHEITGEVIEKGRDVENLQIGDLVSVPF NVACGRCRSCKEMHTGVCLTVNPARAGGAYGYVDMGDWTGGQAEYLLVPY ADFNLLKLPDRDKAMEKIRDLTCLSDILPTGYHGAVTAGVGPGSTVYVAG AGPVGLAAAASARLLGAAVVIVGDLNPARLAHAKAQGFEIADLSLDTPLH EQIAALLGEPEVDCAVDAVGFEARGHGHEGAKHEAPATVLNSLMQVTRVA GKIGIPGLYVTEDPGAVDAAAKIGSLSIRFGLGWAKSHSFHTGQTPVMKY NRALMQAIMWDRINIAEVVGVQVISLDDAPRGYGEFDAGVPKKFVIDPHK TFSAA -
Predicted molecular weight
43 kDa including tags -
Amino acids
2 to 399 -
Tags
His tag N-Terminus -
Additional sequence information
From Pseudomonas putida. Genbank: D21201
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant fdhA protein (ab198463)
4-20% SDS-PAGE analysis of ab198463 with Coomassie staining.
Lane 1: 2.88 µg ab198463
Lane 2: Protein marker -
Functional Studies - Recombinant fdhA protein (ab198463)Kinetics of ab198463.