Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
Key features and details
- Mouse monoclonal [9F12] to p38 alpha/MAPK14
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-p38 alpha/MAPK14 antibody [9F12]
See all p38 alpha/MAPK14 primary antibodies -
Description
Mouse monoclonal [9F12] to p38 alpha/MAPK14 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Dog, Chimpanzee -
Immunogen
Recombinant full length protein corresponding to Human p38 alpha/MAPK14 aa 1 to the C-terminus. (Expressed in E.coli) (NP_001306.1).
Database link: Q16539-2 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: A431, A549, HCT-116, HeLa, HEK-293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3, T98G cell lysates; Rat brain lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9F12 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461) at 1/1000 dilution
Lane 1 :Recombinant Human p38 beta/MAPK11 protein (ab117219)
Lane 2 :Recombinant Human p38 gamma/MAPK12 protein (ab117221)
Lane 3 :Recombinant Human p38 delta/MAPK13 protein (ab113869)
Lane 4 :Recombinant human p38 alpha/MAPK14 protein (ab82188)
Predicted band size: 41 kDa -
All lanes : Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : Hl-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Sheep Anti-Mouse IgG: HRP
Predicted band size: 41 kDa
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Paraffin-embedded human colon carcinoma tissue stained for p38 using ab59461 at 1/10000 dilution in immunohistochemical analysis. Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.