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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)

Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [9F12] to p38 alpha/MAPK14
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-p38 alpha/MAPK14 antibody [9F12]
    See all p38 alpha/MAPK14 primary antibodies
  • Description

    Mouse monoclonal [9F12] to p38 alpha/MAPK14
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Dog, Chimpanzee
  • Immunogen

    Recombinant full length protein corresponding to Human p38 alpha/MAPK14 aa 1 to the C-terminus. (Expressed in E.coli) (NP_001306.1).
    Database link: Q16539-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human colon carcinoma tissue. WB: A431, A549, HCT-116, HeLa, HEK-293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3, T98G cell lysates; Rat brain lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    9F12
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Kits/ Lysates/ Other
    • Kits
    • Cell Signaling Kits
    • Other Cell Signaling Kits

Images

  • Western blot - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
    Western blot - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
    All lanes : Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461) at 1/1000 dilution

    Lane 1 : Recombinant Human p38 beta/MAPK11 protein (ab117219)
    Lane 2 : Recombinant Human p38 gamma/MAPK12 protein (ab117221)
    Lane 3 : Recombinant Human p38 delta/MAPK13 protein (ab113869)
    Lane 4 : Recombinant human p38 alpha/MAPK14 protein (ab82188)

    Predicted band size: 41 kDa

  • Western blot - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
    Western blot - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
    All lanes : Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 7 : Hl-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
    Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 12 : T98G cell lysate
    Lane 13 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Sheep Anti-Mouse IgG: HRP

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 alpha/MAPK14 antibody [9F12] (ab59461)

    Paraffin-embedded human colon carcinoma tissue stained for p38 using ab59461 at 1/10000 dilution in immunohistochemical analysis. Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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