Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)
![Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)](https://www.abcam.com/ps/products/33/ab33910/Images/ab33910-261562-anti-map4k1-antibody-ep630y-western-blot.jpg "Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)")
Key features and details
- Rabbit monoclonal [EP630Y] to MAP4K1/HPK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4K1/HPK1 antibody [EP630Y]
See all MAP4K1/HPK1 primary antibodies -
Description
Rabbit monoclonal [EP630Y] to MAP4K1/HPK1 -
Host species
Rabbit -
Specificity
ab33910 recognises hematopoietic progenitor kinase 1 (HPK1). -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MAP4K1/HPK1 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MAP4K1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.09% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP630Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)All lanes : Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 97 kDaBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)](https://www.abcam.com/ps/products/33/ab33910/Images/ab33910-261568-anti-map4k1-antibody-ep630y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
