Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E239] to RSK1 p90 (phospho S380) - BSA and Azide free
  • Suitable for: ICC, WB, Dot blot, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free
    See all RSK1 p90 primary antibodies

  • Description

    Rabbit monoclonal [E239] to RSK1 p90 (phospho S380) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects RSK1 phosphorylated on Serine 380. 

  • Tested applications

    Suitable for: ICC, WB, Dot blot, IPmore details
    Unsuitable for: Flow Cyt or IHC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247241 is the carrier-free version of ab32203. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247241 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/5000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates.
    Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab32203, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    Immunocytochemistry - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    This data was developed using ab32203, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A431(Human epidermoid carcinoma cell line) cells, treated and non-treated, labeling RSK1 p90 (phospho S380) with Ab32203 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green). The green staining was increased in the EGF(ab9697 100ng/ml, 10min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signaling was obviously decreased. For the pan antibody, there was no great difference after EGF (100ng/ml, 10min) or EGF (100ng/ml, 10min) + LP treatment. The data showed mostly Cytoplasm staining.
  • Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

    This data was developed using ab32203&

    44; the same antibody clone in a different buffer formulation.

    >RSK1 p90 (phospho S380) was immunoprecipitated from 10µg A431 (human epidermoid carcinoma) whole cell lysate with ab32203 at 1/20 dilution (0.5µg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab32203 at 1/200 dilution (0.54 µg/ml). VeriBlot for IP Detection Reagent (HRP) ab131366, was used for detection at 1/1000 dilution.

    Lane 1 Input: A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate 10µg

    Lane 2 (+): ab32203 + A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32203 in A431 (human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    This data was developed using ab32203, the same antibody clone in a different buffer formulation.Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with ab32203 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)
    Anti-RSK1 p90 (phospho S380) antibody [E239] - BSA and Azide free (ab247241)

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