Anti-PHD1/prolyl hydroxylase antibody (ab80361)
Key features and details
- Rabbit polyclonal to PHD1/prolyl hydroxylase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PHD1/prolyl hydroxylase antibody
See all PHD1/prolyl hydroxylase primary antibodies -
Description
Rabbit polyclonal to PHD1/prolyl hydroxylase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human PHD1/prolyl hydroxylase aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab91085) -
Positive control
- This antibody gave a positive signal in MDA-MB231 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications OrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
-
Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity. -
Tissue specificity
Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. -
Sequence similarities
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 112398 Human
- Omim: 606424 Human
- SwissProt: Q96KS0 Human
- Unigene: 515417 Human
-
Alternative names
- DKFZp434E026 antibody
- EGL nine (C.elegans) homolog 2 antibody
- Egl nine homolog 2 (C. elegans) antibody
see all
Images
-
Western blot - Anti-PHD1/prolyl hydroxylase antibody (ab80361)Anti-PHD1/prolyl hydroxylase antibody (ab80361) at 1 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 37 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody (ab80361)ICC/IF image of ab80361 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab80361, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Datasheets and documents
References (2)
ab80361 has been referenced in 2 publications.
- Wang Y et al. Differential effects of sulforaphane in regulation of angiogenesis in a co-culture model of endothelial cells and pericytes. Oncol Rep 37:2905-2912 (2017). PubMed: 28405689
- Chan MC et al. Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. J Biol Chem 291:20661-73 (2016). Human . PubMed: 27502280
Images
-
Western blot - Anti-PHD1/prolyl hydroxylase antibody (ab80361)Anti-PHD1/prolyl hydroxylase antibody (ab80361) at 1 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 37 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody (ab80361)ICC/IF image of ab80361 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab80361, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
