Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies

  • Description

    Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for human Rsk 2 / MAPKAP Kinase 1b.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (C terminal). The exact sequence is proprietary.

  • Epitope

    ab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b.

  • Positive control

    • MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Y82

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
    Lane 3: MCF7 cell lysate (40 µg)
    Lane 4: HeLa cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

    ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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