Recombinant human MMP13 protein (Active) (ab227435)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human MMP13 protein (Active)
See all MMP13 proteins and peptides -
Biological activity
This enzyme has a specific activity of ≥200 mU/mg based on its ability to hydrolyze a FRET-based MMP-13 quenched substrate and results in increase of fluorescence, which can be detected at Ex/Em = 325/393 nm.
One unit is the amount of enzyme that will hydrolyze 1.0 µmole of MMP-13 substrate per minute at pH 7.5 and 37ºC.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
YNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNF TRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDD DETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFM LPDDDVQGIQSLYGPGDEDPN -
Predicted molecular weight
21 kDa including tags -
Amino acids
104 to 274 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Proprietary buffer.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute the lyophilized protein in 30% glycerol to a final concentration of 1.0 mg/ml and incubate the reconstituted protein at 25 °C for 15 minutes. Store in working aliquots at -20°C.
Images
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Functional Studies - Recombinant human MMP13 protein (Active) (ab227435)
The activity of ab227435 is ≥ 200 mU/mg based on its ability to hydrolyze a FRET-based MMP-13 substrate.
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SDS-PAGE - Recombinant human MMP13 protein (Active) (ab227435)4-20% SDS-PAGE analysis of 5 µg ab227435.
Recombinant protein loaded under reducing conditions and stained with Coomassie Blue.
The protein shows a predicted MW of ~ 20.5 kDa.
