Mouse MMP12 ELISA Kit (ab246540)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 32.858 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MMP12 ELISA Kit
See all MMP12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 8 2% Inter-assay Sample n Mean SD CV% supernatant 3 3.5% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32.858 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 80% - 84% Tissue Extracts 86 84% - 89% Tissue Culture Media 87 84% - 91% Hep Plasma 88 85% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MMP12 ELISA Kit (ab246540) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP12 protein in serum, cell culture extracts, tissue extracts, cell culture supernatant, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP12 with 32.858 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MMP12 Capture Antibody 1 x 600µl 10X Mouse MMP12 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse MMP12 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- EC 3.4.24.65
- HME
- Macrophage elastase
see all -
Database links
- Entrez Gene: 17381 Mouse
- SwissProt: P34960 Mouse
- Unigene: 2055 Mouse
Images
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Other - Mouse MMP12 ELISA Kit (ab246540)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MMP12 standard curve in Sample Diluent NS.The MMP12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse MMP12 standard curve in 1X Cell Extraction Buffer PTR.The MMP12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MMP12 in mouse lung and spleen tissue culture supernatant samples.The concentrations of MMP12 were measured in duplicates, interpolated from the MMP12 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 25% and spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP12 concentration was determined to be 22.57 ng/mL in lung supernatant and 5.77 ng/mL in and spleen supernatant.
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. Interpolated concentrations of native MMP12 in mouse lung extract based on a 4,000 µg/mL extract load.The concentrations of MMP12 were measured in duplicate and interpolated from the MMP12 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP12 concentration was determined to be 788.83 pg/mL in mouse lung extract.
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Sandwich ELISA - Mouse MMP12 ELISA Kit (ab246540)To learn more about the advantages of recombinant antibodies see here.
