Anti-Pit1 antibody [5E4] (ab10545)
Key features and details
- Mouse monoclonal [5E4] to Pit1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Rat
- Isotype: IgG2a
Overview
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Product name
Anti-Pit1 antibody [5E4]
See all Pit1 primary antibodies -
Description
Mouse monoclonal [5E4] to Pit1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat -
Immunogen
Recombinant fragment:
SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL
, corresponding to amino acids 30-146 of Rat Pit1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep. -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5E4 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1. -
IF using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.
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Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.