Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pit1 antibody [5E4] (ab10545)

Key features and details

  • Mouse monoclonal [5E4] to Pit1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Rat
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Pit1 antibody [5E4]
    See all Pit1 primary antibodies

  • Description

    Mouse monoclonal [5E4] to Pit1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment:

    SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL

    , corresponding to amino acids 30-146 of Rat Pit1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
    Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep.
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    5E4

  • Myeloma

    NS1

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Pit1 antibody [5E4] (ab10545)
    Western blot - Anti-Pit1 antibody [5E4] (ab10545)

    At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

    At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
  • Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)
    Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545) This image is courtesy of Zelton Dave Sharp
    IF using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.
  • Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)
    Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)
    Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Pit1 antibody [5E4] (ab10545)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.