Anti-DjC8 antibody (ab86138)
Key features and details
- Rabbit polyclonal to DjC8
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DjC8 antibody
See all DjC8 primary antibodies -
Description
Rabbit polyclonal to DjC8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).
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Positive control
- Whole cell lysate from HeLa cells and 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DjC8 antibody (ab86138)All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DjC8 antibody (ab86138)Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
