Anti-IKK gamma/NEMO (phospho S85) antibody (ab63551)
Key features and details
- Rabbit polyclonal to IKK gamma/NEMO (phospho S85)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IKK gamma/NEMO (phospho S85) antibody
See all IKK gamma/NEMO primary antibodies -
Description
Rabbit polyclonal to IKK gamma/NEMO (phospho S85) -
Host species
Rabbit -
Specificity
Detects endogenous levels of IKK gamma/NEMO only when phosphorylated at serine 85.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IKK gamma/NEMO (phospho S85).
Sequence:Q-A-Sp-Q-R
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Positive control
- Extracts from HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IKK gamma/NEMO (phospho S85) antibody (ab63551)All lanes : Anti-IKK gamma/NEMO (phospho S85) antibody (ab63551) at 1/500 dilution
Lane 1 : extracts from HepG2 cells,
treated with Anisomycin (0.5uM, 5hours)
Lane 2 : extracts from HepG2 cells,
treated with Anisomycin (0.5uM, 5hours) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
