Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16976] to SRSF3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SRSF3 antibody [EPR16976] - BSA and Azide free
    See all SRSF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16976] to SRSF3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251252 is the carrier-free version of ab198291 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251252 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SFRS3

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16976

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    All lanes : Anti-SRSF3 antibody [EPR16976] (ab198291) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    This data was developed using ab198291, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Immunocytochemistry - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using ab198291, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) labelling SRSF3 with purified ab198291 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    All lanes : Anti-SRSF3 antibody [EPR16976] (ab198291) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor) whole cell lysate
    Lane 4 : RAW 264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    This data was developed using ab198291, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using ab198291, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    This data was developed using ab198291, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    This data was developed using ab198291, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Flow Cytometry - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    This data was developed using ab198291, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T Cell Leukemia cells from peripheral blood) labeling SRSF3 with ab198291 at 1/440 dilution (red) compared with a Rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)
    Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

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