Anti-DHX8/prp22 antibody (ab70588)
Key features and details
- Rabbit polyclonal to DHX8/prp22
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DHX8/prp22 antibody
See all DHX8/prp22 primary antibodies -
Description
Rabbit polyclonal to DHX8/prp22 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human DHX8/prp22 aa 325-375.
Database link: NP_004932.1 -
Positive control
- Whole cell lysate from HeLa and 293T cells.
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General notes
This product was previously labelled as DHX8
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (ab70588)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8/prp22 with ab70588 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-DHX8/prp22 antibody (ab70588) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at:
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Immunoprecipitation of DHX8/prp22 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.