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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Anti-DHX8/prp22 antibody (ab70588)

Anti-DHX8/prp22 antibody (ab70588)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DHX8/prp22
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DHX8/prp22 antibody
    See all DHX8/prp22 primary antibodies
  • Description

    Rabbit polyclonal to DHX8/prp22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human DHX8/prp22 aa 325-375.
    Database link: NP_004932.1

  • Positive control

    • Whole cell lysate from HeLa and 293T cells.
  • General notes

     This product was previously labelled as DHX8

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (ab70588)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (ab70588)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8/prp22 with ab70588 at 1/200 (1µg/ml). Detection: DAB.

  • Western blot - Anti-DHX8/prp22 antibody (ab70588)
    Western blot - Anti-DHX8/prp22 antibody (ab70588)
    All lanes : Anti-DHX8/prp22 antibody (ab70588) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 139 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?
    Additional bands at:
  • Immunoprecipitation - Anti-DHX8/prp22 antibody (ab70588)
    Immunoprecipitation - Anti-DHX8/prp22 antibody (ab70588)

    Immunoprecipitation of DHX8/prp22 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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    Applications: WB

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