Anti-ME2 antibody - C-terminal (ab229471)
Key features and details
- Rabbit polyclonal to ME2 - C-terminal
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ME2 antibody - C-terminal
See all ME2 primary antibodies -
Description
Rabbit polyclonal to ME2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human ME2 (C terminal). The exact sequence is proprietary.
Database link: P23368 -
Positive control
- WB: THP-1 and HL-60 whole cell extracts. IP: THP-1 whole cell extract. IHC-P: Mouse muscle tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ME2 antibody - C-terminal (ab229471)All lanes : Anti-ME2 antibody - C-terminal (ab229471) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 2 : HL-60 (human romyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-ME2 antibody - C-terminal (ab229471)ME2 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extract with 5 µg ab229471. Western blot was performed from the immunoprecipitate using ab229471. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: THP-1 whole cell extract.
Lane 2: Control IgG IP in THP-1 whole cell extract.
Lane 3: ab229471 IP in THP-1 whole cell extract. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody - C-terminal (ab229471)Paraffin-embedded mouse muscle tissue stained for ME2 using ab229471 at 1/500 dilution in immunohistochemical analysis.
