Anti-ME2 antibody (ab233509)
Key features and details
- Rabbit polyclonal to ME2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ME2 antibody
See all ME2 primary antibodies -
Description
Rabbit polyclonal to ME2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human ME2 aa 200-450. Expressed in E. coli. N-terminal tags.
Database link: P23368 -
Positive control
- WB: Recombinant human ME2 protein; Human lung tissue lysate; Jurkat, K562 and HeLa whole cell lysate (ab150035). IHC-P: Human cerebrum, liver, glioma, stomach, kidney and pancreatic cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ME2 antibody (ab233509) at 1 µg/ml
Lane 1 : Human lung tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for ME2 using ab233509 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Anti-ME2 antibody (ab233509) at 2 µg/ml + Recombinant human ME2 protein