Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (ab268058)
Key features and details
- Mouse monoclonal [CPTC-AKR1B1-2] to Aldose reductase
- Suitable for: WB, IHC-P, Protein Array, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Aldose reductase antibody [CPTC-AKR1B1-2]
See all Aldose reductase primary antibodies -
Description
Mouse monoclonal [CPTC-AKR1B1-2] to Aldose reductase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human Aldose reductase aa 1-316.
Database link: P15121 -
Positive control
- WB: Hela cell lysate. ICH/ IF: A549 cells. IHC: Human prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
CPTC-AKR1B1-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human Prostate stained for Aldose reductase using ab268058 at 2 μg/ml in immunohistochemical analysis.
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Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (ab268058) at 2 µg/ml + HeLa cell lysate
Predicted band size: 35 kDa
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Immunofluorescence staining of A549 cells labeling Aldose reductase with ab268058 at 2 ug/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Blue)
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Protein Array containing more than 19,000 full-length human proteins using ab268058. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.