Anti-Aldose reductase antibody (ab62795)
Key features and details
- Rabbit polyclonal to Aldose reductase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Aldose reductase antibody
See all Aldose reductase primary antibodies -
Description
Rabbit polyclonal to Aldose reductase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Aldose reductase aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab92665)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications OrangutanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
-
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. -
Tissue specificity
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 231 Human
- Omim: 103880 Human
- SwissProt: P15121 Human
- Unigene: 521212 Human
-
Alternative names
- ADR antibody
- AKR1B 1 antibody
- Akr1b1 antibody
see all
Images
-
Western blot - Anti-Aldose reductase antibody (ab62795)All lanes : Anti-Aldose reductase antibody (ab62795) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human placenta tissue lysate - total protein (ab29745)
Lane 7 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab62795)ICC/IF image of Aldose reductase stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (2)
ab62795 has been referenced in 2 publications.
- Ramos RJ et al. Discovery of pyridoxal reductase activity as part of human vitamin B6 metabolism. Biochim Biophys Acta Gen Subj 1863:1088-1097 (2019). PubMed: 30928491
- Hevir N et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). IHC-P, WB ; Human . PubMed: 23146748
Images
-
Western blot - Anti-Aldose reductase antibody (ab62795)All lanes : Anti-Aldose reductase antibody (ab62795) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human placenta tissue lysate - total protein (ab29745)
Lane 7 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab62795)
ICC/IF image of Aldose reductase stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
