Anti-Aldose reductase antibody (ab153897)
Key features and details
- Rabbit polyclonal to Aldose reductase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aldose reductase antibody
See all Aldose reductase primary antibodies -
Description
Rabbit polyclonal to Aldose reductase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human Aldose reductase aa 1-316.
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Positive control
- 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Aldose reductase antibody (ab153897) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Aldose reductase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
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All lanes : Anti-Aldose reductase antibody (ab153897) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Aldose reductase with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Aldose reductase with ab153897 at 1/500 dilution.