Anti-Ly6c antibody [ER-MP20] (ab15627)
![Anti-Ly6c antibody [ER-MP20] (ab15627)](https://www.abcam.com/ps/products/15/ab15627/Images/ab15627-273484-anti-ly6c-antibody-er-mp20-immunohistochemistry.jpg "Anti-Ly6c antibody [ER-MP20] (ab15627)")
Key features and details
- Rat monoclonal [ER-MP20] to Ly6c
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Ly6c antibody [ER-MP20]
See all Ly6c primary antibodies -
Description
Rat monoclonal [ER-MP20] to Ly6c -
Host species
Rat -
Specificity
ab15627 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
Database link: P0CW02 -
Epitope
Spleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line. -
Positive control
- Monoblasts, late CFU M cells, monocytes, granulocytes. Macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs. Activated macrophages in inflammatory tissues.
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General notes
ab15627 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15627 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 99% PBS, 0.6% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
Primary antibody notes
CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. ab15627 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15627 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells. -
Clonality
Monoclonal -
Clone number
ER-MP20 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [ER-MP20] (ab15627)
ab15627 staining Ly6c in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by proteinase K pretreatment. Samples were incubated with primary antibody (1/400).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [ER-MP20] (ab15627)](https://www.abcam.com/ps/products/15/ab15627/Images/ab15627-249769-anti-ly6c-antibody-er-mp20-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [ER-MP20] (ab15627) This image is courtesy of an anonymous Abreviewab15627 staining Ly6c in Mouse tumor stroma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeablizied with Tween-20 and blocked with 1% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate pH6. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. A Avidin / Streptovidin amplification kit was used followed by DAB development.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [ER-MP20] (ab15627)](https://www.abcam.com/ps/products/15/ab15627/Images/ab15627-23291-anti-ly6c-antibody-er-mp20-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [ER-MP20] (ab15627) Image from Lu X et al, J Biol Chem. 2009 Oct 16;284(42):29087-96. Epub 2009 Aug 31, Fig 2. DOI 10.1074/jbc.M109.035899ab15627 staining Ly6c in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin for hematoxylin and eosin staining. Immunohistochemical analysis was performed with heat-induced antigen retrieval. Biotinylated secondary antibody was used with an ABC kit and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin.
Scale bar 200 µm.
