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Immunology Innate Immunity Macrophage / Inflamm.

Biotin Anti-Ly6c antibody [ER-MP20] (ab15674)

Price and availability

268 032 ₸

Availability

Order now and get it on Thursday February 25, 2021

Biotin Anti-Ly6c antibody [ER-MP20] (ab15674)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Biotin Rat monoclonal [ER-MP20] to Ly6c
  • Suitable for: IHC-Fr
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG2a

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Overview

  • Product name

    Biotin Anti-Ly6c antibody [ER-MP20]
    See all Ly6c primary antibodies
  • Description

    Biotin Rat monoclonal [ER-MP20] to Ly6c
  • Host species

    Rat
  • Conjugation

    Biotin
  • Specificity

    ab15674 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended.
  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
    Database link: P0CW02

  • Epitope

    Spleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line.
  • Positive control

    • Monoblasts, late CFU M cells, monocytes, granulocytes. Macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs. Activated macrophages in inflammatory tissues.
  • General notes

    ab15674 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% KATHON™ CG/ICP
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  • Primary antibody notes

    ab15674 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.
  • Clonality

    Monoclonal
  • Clone number

    ER-MP20
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Immunology
    • Innate Immunity
    • Complement
    • MAC
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Images

  • Immunohistochemistry (Frozen sections) - Biotin Anti-Ly6c antibody [ER-MP20] (ab15674)
    Immunohistochemistry (Frozen sections) - Biotin Anti-Ly6c antibody [ER-MP20] (ab15674)

    ab15674 (1/1000) staining Ly6c in frozen mouse speen. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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