Biotin Anti-Ly6c antibody [ER-MP20] (ab15674)
Key features and details
- Biotin Rat monoclonal [ER-MP20] to Ly6c
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Ly6c antibody [ER-MP20]
See all Ly6c primary antibodies -
Description
Biotin Rat monoclonal [ER-MP20] to Ly6c -
Host species
Rat -
Conjugation
Biotin -
Specificity
ab15674 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
Database link: P0CW02 -
Epitope
Spleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line. -
Positive control
- Monoblasts, late CFU M cells, monocytes, granulocytes. Macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs. Activated macrophages in inflammatory tissues.
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General notes
ab15674 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 99% PBS, 0.5% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
Primary antibody notes
ab15674 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells. -
Clonality
Monoclonal -
Clone number
ER-MP20 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2a -
Research areas