Anti-Ly6c antibody (ab77766)
Key features and details
- Rabbit polyclonal to Ly6c
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Ly6c antibody
See all Ly6c primary antibodies -
Description
Rabbit polyclonal to Ly6c -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Ly6c aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab96115) -
Positive control
- This antibody gave a positive signal in Mouse thymus tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).Target
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Relevance
Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor. -
Database links
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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Alternative names
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
Images
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Western blot - Anti-Ly6c antibody (ab77766)Anti-Ly6c antibody (ab77766) at 1 µg/ml + Thymus (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
We hypothesize that the 11 kDa represents the mature form of Mouse Lymphocyte antigen 6C precursor. Ly6c has a 26 amino acid signal peptide which, when cleaved, gives an expected molecular weight of 11 kDa.
Datasheets and documents
References (1)
ab77766 has been referenced in 1 publication.
- MohanKumar K et al. A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions. Nat Commun 10:3494 (2019). PubMed: 31375667
Images
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Western blot - Anti-Ly6c antibody (ab77766)Anti-Ly6c antibody (ab77766) at 1 µg/ml + Thymus (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
We hypothesize that the 11 kDa represents the mature form of Mouse Lymphocyte antigen 6C precursor. Ly6c has a 26 amino acid signal peptide which, when cleaved, gives an expected molecular weight of 11 kDa.
